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Quantitative accuracy is enhanced through incorporation of stable isotope-labeled synthetic peptides as internal standards (Liquid Chromatography-Stable Isotope Dilution-MRM MS or LC-SID-MRM MS), a methodology successfully applied for absolute quantification of CFTR protein using signature peptides as full-length protein surrogates (McShane et al., 2014)

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Subsequently, 5 mL of 0.5 M H 2 O 2 was included in the in-situ cell, and we collected the in-situ FTIR spectra at specific intervals, maintaining a reaction time of 10 min

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Mato et al., 2005)