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This step removes positively charged trypsinized peptides from the sample, while negatively charged peptides (e.g., phosphopeptides) are collected in the early eluting fractions

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Liver toxicity would require sustained copper intake far exceeding normal use

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The pellet was resuspended in 2 ml measurement buffer (0.1 M Sorbitol, 0.1 M NaCl, 0.1 M TrisHCl pH 7.4) and split into 4 500 l aliquots for the measurement of the steady state, the oxidized and reduced controls, and the recovery experiment, respectively