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The hemibrain pellets were immediately lysed in 100 L of 1% SDS buffer (1% SDS in IP Lysis Buffer supplemented with 1X protease inhibitor, 1X phosphatase inhibitor, 100 mM N-Ethylmalamide), and homogenized with a pestle then boiled at 95 C for 10 min

Important caveat: the combination is not clinically validated

Visceral fat measurements show meaningful reduction

Udovin, L

Mukherjee S, Madamsetty VS, Bhattacharya D, Roy Chowdhury S, Paul MK, Mukherjee A (2020) Recent advancements of nanomedicine in neurodegenerative disorders theranostics

Samples with missing data in key covariates were removed prior to batch correction, leaving 124 samples