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In vitro succinylation assay Purified GCLC proteins were incubated with different concentrations of succinyl-CoA (S1129, Sigma-Aldrich) in TBS buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl) at 37 C for 4 h

Studies have shown its effectiveness in reducing the survival of senescent lung fibroblasts and umbilical vein endothelial cells [102]

To reduce these adverse effects in oocytes and embryos, various antioxidants had been applied in IVF ( The pregnancy rate in IVF/ICSI-ET cycles is closely related to the quality of transplanted embryos and the endometrial receptivity to embryos, especially the former ( in vitro and during oocyte maturation ( in vitro

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