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Note: unlike many other kinases, such as those involved in regulating glycogen metabolism, pyruvate dehydrogenase kinase is not regulated by cAMP levels, but rather by molecules that signal changes in the energy status of the cell and the availability of biosynthetic intermediates, ATP and NADH, and acetyl-CoA, respectively

10.1016/j.freeradbiomed.2018.09.043 50 IngoldI.BerndtC.SchmittS.DollS.PoschmannG.BudayK.et al (2018)

SCD1 facilitates conversion of saturated fatty acids to monounsaturated fatty acids (MUFAs), while polyunsaturated fatty acids (PUFAs) accumulate

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After 1 mM CaCl 2 was added for Tse3-Tsi3 protein interaction, the supernatant was then loaded to the CNBr-activated Sepharose resin (GE Healthcare) conjugated with Tse3 protein by gravity flow