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While this usually does not pose a significant problem,it may lead to an incorrect estimation of the tagged proteins molecular mass.A further drawback of his-tag-based purification is the co-education of endogenous protein that naturally possess two or more adjacent histidine residues

doi: 10.1073/pnas.042707999 75 EmanueleS.CelesiaA.DAnneoA.LauricellaM.CarlisiD.De BlasioA.et al

Subsequently, 20 l (corresponding roughly to 2.5 g) of the desalted protein was mixed with 30 l of 100 mM HEPES p H 7.0 buffer containing different redox agents at a final concentration of 10 mM

[PMC free article] [PubMed] [Google Scholar] Andreazza AC, Duong A, Young LT

Identity-by-descent relatedness analysis in Vietnam indicated high parasite connectivity between coastal provinces, and a distinct population, with high levels of relatedness, in a remote highland province

Skalny AA, Tinkov AA, Medvedeva YS, Alchinova IB, Karganov MY, Skalny AV et al (2015) Effect of short-term zinc supplementation on zinc and selenium tissue distribution and serum antioxidant enzymes
