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In the nematode Caenorhabditis elegans, the nuclear receptor DAF-12 acts as a dietary and environmental sensor to orchestrate diverse aspects of development, metabolism, and reproduction

To expand on the chemistry of attaching ligands to agarose beads a little, there are usually 3 components to the ligand (Figure 3): the ligand itself that binds to the peptide or protein tag the reactive chemical group that forms a covalent bond with the ligand a spacer that connects the reactive chemical group to the agarose bead Figure 3