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Then the calibration curve was plotted using absorbance obtained against different concentrations

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Reactive astrogliosis in neuroinfection is thus defensive and neuroprotective: inhibition of astrocytic reactivity, for example by knocking out GFAP, 231 or by genetic deletion of NK-1R receptor for substance P, which suppresses reactive astrogliosis that defines resistance to N

It involves freezing the peptide and then reducing the surrounding pressure to allow the frozen water in the material to sublimate directly from a solid to a gas

However, measures of grey matter atrophy are less useful in differentiating between patients with PD with different disease stages or outcomes: some studies have shown greater volume loss within right thalamus, hippocampus and fronto-temporal regions in PD with mild cognitive impairment (MCI) compared to those with intact cognition 122,123 but this was not replicated in a recent multicentre study that only found differences with behavioural symptoms but not cognition at 1 year follow-up 124

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