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Protein was eluted with 4CV of 60% buffer B and immediately diluted 10-fold with Buffer A prior to concentration and 3 rounds of buffer exchange to remove excess imidazole by centrifugation at 3095xg at 4 C in 100 kDa cutoff concentrators (Amicon)
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A., Ahmad, R., Halim, A
A lack of sleep has been linked to lower testosterone levels in young and older men
BCL-2 and glutathione: alterations in cellular redox state that regulate apoptosis sensitivity
