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Oxidative stress is the condition whereby the living organism is unable to cope with an excessive production of free radicals and reactive electrophilic species generated within the cellular metabolism as a consequence of exposure to several chemicals, among which some metals [10,11]

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Therefore, maintaining a balanced equilibrium between ROS and antioxidants is essential, as is a careful assessment of oxidative stress levels in the body before recommending antioxidant use [2]

In a tube, 100 L of 1.2% benzoic acid and 1.4 ml H 2 O were mixed with 100 L of the sample, and the solution was filtered through a 0.22-m filter cartridge into a 4-ml glass vial