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Your calculated dose is accurate based on what went into the vial, but the actual active compound is lower because some percentage was destroyed by shaking

Externally introduced arginase analogs can inhibit T cells antitumor activity by reducing the arginine level in the tumor microenvironment, just as the tumor or immunosuppressive cells expressing arginase 1 can

Diabetologia 47 , 20122021, doi: 10.1007/s00125-004-1570-9 (2004)

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Using this strategy, Niu et al

Working through the calculation carefully prevents the dosing errors that compromise research validity