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Before sectioning, all the equipment and samples were left inside the cryostat for about 5 minutes for temperature adjustment

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Patients who become infected with HCV will typically develop abnormal laboratory findings in the following order: detectable HCV RNA, followed by elevation in ALT, and then anti-HCV (Figure 2).[8,25] Patients who develop a clinical illness with acute HCV infection usually have onset of symptoms well after the onset of viremia, but soon after, or concomitant with, increases in ALT levels

Circulation 124(10):11511159

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doi:10.1002/14651858.CD009996.pub2