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The amplification of miRNA cDNAs was quantified in real-time SYBR Green RT-qPCR reactions according to the recommended two-step cycling procedure (preincubation/activation at 95 C for 2 min followed by 40 cycles of 95 C for 5 s, 60 C for the 30 s)

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Jr Cellular, but not matrix, immunolocalization of SPARC in the human intervertebral disc: decreasing localization with aging and disc degeneration

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