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Our computations indicate that the method to compute the proton solvation energy is robust with respect to the detailed computational setup and the construction of the solvation model

Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

doi: 10.1002/bies.202300055

This article describes the published research literature on these two peptides for orientation purposes within the UK research community

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