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ROS and nitrogen species are synthesized at low levels in skeletal muscle cells and are required for normal force production

Alsene, K., Deckert, J., Sand, P., & de Wit, H

Endocrinology and metabolism 282,2 (2002): E474-82

After permeabilization treatment, the culture broth was centrifuged and cells were resuspended in the biotransformation medium (crotonobetaine in phosphate buffer) for 48 h