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Primary antibodies (Caspase-3 1:500, Bax 1:500, TGF- 1:100, and IGF-1 1:500) were incubated overnight at 4C

USA 106 , 1679916804 (2009)

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Statistics and reproducibility Statistical significance was determined using independent t -test for immunohistochemical and western blot analyses, and one-way analysis of variance for cell cycle and apoptosis detection by GraphPad Prism 7.0 software (GraphPad, Inc., La Jolla, CA, USA)

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