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Enzyme-linked immunosorbent assay (ELISA) Pierce TM Streptavidin Coated Clear 96-Well plates (Thermo Fisher Scientific, Dreieich, Germany) were incubated with 90 L of antigen solution per well at 25 C for 45 min, shaking on a Thermomixer compact (Eppendorf, Hamburg, Germany) at 350 RPM

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