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cysteine and glutathione reaction GSH hoards all the cysteine—what a slimy thing to do Frontiers | Cysteine Depletion, a
Description
Other suitable methods are known in the art and the methods are not limited to any particular method of ex vivo expansion of the cells

F1000Research , 3 , 157

Allow alcohol to dry completely before injecting

Eur J Neurosci 20:25242540 Gentleman SM, Leclercq PD, Moyes L, Graham DI, Smith C, Griffin WS, Nicoll JA (2004) Long-term intracerebral inflammatory response after traumatic brain injury

The reason is practical: fewer steps mean fewer opportunities for confusion

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