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At the end of the incubation of 30 min or 90 min, some of the MCTS were transferred to a new agarose-coated 96-well plate, and cultured in drug-free medium for different lengths of time (2, 6, 12, and 24 h) to further study the post treatment effects and drug elimination

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10.1016/S0378-4274(99)00160-5 Toxicol

Osteocalcin contains three glutamate residues that are modified by -carboxylation