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[DOI] [PMC free article] [PubMed] [Google Scholar] 47.Powell LW, Dixon JL, Ramm GA, Purdie DM, Lincoln DJ, Anderson GJ, Subramaniam VN, et al

Multiplex immunofluorescence (mIF) and image analysis Sections from FFPE processed explants were stained using Opal 4-Color Manual IHC Kit (Cat# NEL810001KT, Akoya Biosciences, Boston, MA, USA) according to manufacturers instructions

MEK inhibition resulted in an increase in the pMEK signal due to multiple and opposing feedback loops from ERK and RSK

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Next, 100 L of water and chloroform were added and samples were vortexed and centrifuged at 18,787 g for 7 min at 4 C