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doi: 10.3389/fmicb.2024.1337632, 24

After centrifugation at 20,817 g at 4 C for 20 min, the concentrations of cell lysate were measured using Micro BCA Protein Assay Kit (Thermo Fisher Scientific)

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AcAc and BHB are interconverted by an NAD-dependent enzyme (Bdh1), driven both by substrate concentration and by the mitochondrial NAD + /NADH ratio