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Direct-to-biology (D2B) approaches are generally defined as a set of strategies that aim at the biological study of chemical compounds without the need for post-synthesis purification, i.e., biological evaluation of the crude product 183,184

[ 1 , 3 ] Epithalamin was studied extensively through the 1980s and 1990s for its effects on aging, immune function, and melatonin production in animal models and some human trials

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(Princeton, NJ, USA) using a validated liquid chromatography electrospray ionisation with tandem mass spectrometric detection method over the concentration ranges of 102000 and 1000200 000 ng ml 1 (Latz et al, 2006)