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In addition, regions distant from the active center (Figs 3 and 4) show significant structural changes after oxidation (N-terminal end of 3 and 5 )

Cell culture and treatment THP-1 cells (ATCC, TIB-202) were cultured in RPMI-1640 medium (MeilunBio, MA0215) supplemented with 10% FBS and 1% P/S under 37 C and 5% CO 2

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The results revealed that while keeping a delivered dose close to 80%, the captured fugitive aerosol remained between 1.6 and 2.1%

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However, the natural decomposition is slow and thus the resultant low release of nutrients will limit the agronomic benefits of buried animal by-products and carcasses as a nutrient source for crops or soil improvement