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The total number of the cells was evaluated based on the following formula: where Q was the total number of sexual lineage cells observed in all disectors, h was the optical disector's height, a/f was the area of the counting frame, P was the total number of observed frames, BA was the setting of the microtome, and t was the mean of the final section thickness [36]

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DNA Extraction and Genotyping Genomic DNA was extracted from PBMCs of patients using a TIANamp Blood DNA Kit (Tiangen, Beijing, China)

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