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ROIs were either determined manually based on the expression domain of a reporter gene, or were determined with automatic segmentation for all visible nuclei using either TrackMate or Imaris
Integrative analysis of HIF binding and transactivation reveals its role in maintaining histone methylation homeostasis

The concentration of SSA in the sample should be 0.5 to 1% at the time of measurement

However, reduced glutathione is synthesized by both prokaryotic and eukaryotic cells

Nutrients, 11 (3), 649

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