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For migration assays, the conditioned medium was diluted to one half in DMEM medium containing 1% BSA

Samples were then transferred to 0.2 m centrifugal filters and centrifuged again at 10,000 RCF for 5 min at 4C before being transferred to glass auto-sampler vials ready for injection

Abdullah NA, Jaafar F, Yasin HM, Taha H, Petalcorin MIR, Mamit MH, et al

The contribution of metabolites to the rapid and potent down-regulation of rat cortical beta-adrenoceptors by the putative antidepressant sibutramine hydrochloride

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