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The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 445

It is highly expressed in energy-demanding tissues (e.g., heart, brain, skeletal muscle) (Villena, 2015)

Mechanisms include reduction of excitotoxicity and oxidative stress, enhanced neurotrophic factor expression supporting neuronal survival, angiogenesis improving cerebral blood flow, and modulation of dopamine and serotonin systems