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Levels of AST, ALT, creatinine, and oxidative stress markers including TBARS, SOD, CAT, GPx, TAS, TOS, GSH, and GSSG, along with pro-inflammatory cytokines IL-1, IL-6, IL-8, IL-10, IL-17, and TNF-, were measured using ELISA kits

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Nickel affinity chromatography The nickel on agarose beads binds to proteins through polyhistidine tags, known as his-tags, that usually range from 6 to 10 histidine residues long (Figure 2)
