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This marking is accomplished by hydroxylating the proline amino acid residue within HIF-1

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To mitigate this side effect, individuals can rotate injection sites and use proper injection techniques

The second experiment demonstrated that restriction did not have to be initiated in partially grown mice, as males of the same F1 genotype also lived longer on a low methionine diet when initiated a 1 year of age (Sun et al., 2009)

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