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10.1016/j.jped.2023.10.005

Quantitative PCR Total cellular mRNA was extracted using the RNeasy kit (Qiagen) and converted to complementary DNA with a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA, USA)

-Angiogenesis and Endothelial Protection The peptide promotes neovascularization by upregulating VEGFR2 and egr-1 pathways while stabilizing endothelial integrity under oxidative or inflammatory stress 1,2

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Lower urinary tract surgeries, medications that affect neuromuscular activity, and diabetes are among the conditions known to be associated with retrograde ejaculation

Peripheral circadian clocks are diversely affected by adrenalectomy