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Total DNA was extracted using the extraction reagent (DNA zol BD Reagent, Invitrogen) following homogenization

Reconstitute the 1mg lyophilized vial in 10mM hydrochloric acid (HCl) at a concentration of 0.5-1.0 mg/mL to ensure complete solubilization, as IGF-1 LR3's isoelectric point near pH 8.4 makes it poorly soluble in neutral buffers and prone to aggregation at physiological pH
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Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth

Ruethers T, Johnston EB, Karnaneedi S, Nie S, Nugraha R, Taki AC et al (2023) Commercial shellfish skin prick test extracts show critical variability in allergen repertoire
