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An unpaired t-test was used to compare differences in background characteristics between groups

Immunodetection was applied with primary antibodies specific to erythroid 2-related factor 2 (Nrf2) (1:5,000, Affinity, AF0639), phospho-Nrf2 (p-Nrf2) (1:1,000, Affinity, DF7519), superoxide dismutase 1 (SOD1) (1:1,000, Thermo, PA130195), glutathione peroxidase (GPx) (1:1,000, Abcam, Ab22604), catalase (1:2,000, Santa Cruz, sc-271803), NF-B (p-65) (1:250, Santa Cruz, sc-8008), TNF- (1:200, Santa Cruz, sc-52746), IL-6 (1:500, Bioss, bs-0782R-TR), IL-1 (1:100, Santa Cruz, sc-52012), COX2 (1:200, Santa Cruz, sc-376861), activating transcription factor 6 (ATF6) (1:200, Santa Cruz, sc-166659), inositol-requiring enzyme 1 (IRE1) (1:250, Santa Cruz, sc-390960), protein kinase R-like endoplasmic reticulum kinase (PERK) (1:100, Santa Cruz, sc-377400), caspase 3 (1:200, Santa Cruz, sc-56053), and -actin (1:5,000, Novus, NB600-501), respectively

Assessment of dimethylxanthine formation from caffeine in healthy adults: comparison between plasma and saliva concentrations and urinary excretion of metabolites

Metabolic reprograming of anti-tumor immunity

Batcioglu K, Karagozler AA, Genc M, Celik S

In mouse embryonic fibroblasts, the elevation of CHAC1 protein expression following As III exposure is negatively regulated by the Tribbles homolog 3 (TRIB3), which limits CHAC1s pro-death potential (rd et al., 2016)