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-Galactosidase activity assay Cells were washed two times with PBS and fixed in 2% formaldehyde, 0.2% glutaraldehyde in PBS for 5 min
Some hypothesize that dietary treatment may also promote the unlearning of seizures by its effects on gene regulation [56, 58, 69, 70]
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Thus, mutations that significantly impair the function of the enzyme cause VLCADD
