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Sci Adv (2019) 5:eaaw2238

Edited by: Ranke MB, Price DA, Reiter EO

doi: 10.1186/s13098-018-0318-5 247

After the 3 rd passage, cells appear to be stable in culture 29

Discovered in 1993, it is the second most common cause of familial/genetic ALS in those of European ancestry, affecting 12% of patients with familial ALS [7]

Once the needle is in place, gently pull back on the plunger just a tiny bit