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cells were fixed (4% paraformaldehyde), permeabilized (0.5% Triton X-100), and labeled with secondary AlexaFluor 488 goat- -human IgG Ab (Biosource), with 2 cold PBS washing in between steps

J Exp Med 177:17791784 Lowenstein CJ, Alley EW, Raval P, Snowman AM, Snyder SH, Russell SW, Murphy WJ (1993) Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide

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Acknowledgements This work was supported by the Ministry of Food and Drug Safety (grant No