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This phenomenon was first observed in a 2013 study by Pasquier et al., which demonstrated that endothelial cells preferentially transfer mitochondria to cancer cells via tunneling nanotubes, leading to enhanced chemoresistance by promoting mitochondrial acquisition and survival advantages in recipient tumor cells [466]

Save a small aliquot from each step of the purification for analysis by SDS-PAGE to monitor the location of the fusion protein throughout the purification (see Notes 13 and 14)

Tominaga, K., Hongo, N., Fujishita, M., Takahashi, Y., & Adachi, Y

[DOI] [PMC free article] [PubMed] [Google Scholar] 89.Ploplis VA, Cornelissen I, Sandoval-Cooper MJ, Weeks L, Noria FA, Castellino FJ

However, there are some things to keep in mind: Allergic Reactions: May be seen rarely in sensitive individuals

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