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The supernatant was removed and total protein concentration in the soluble fraction was determined using a NanoDrop UV spectrometer (Thermo Fisher Scientific) before being transferred to standard chromatography vials with polypropylene inserts (Agilent Technologies) and kept at 4C on a Peltier cooler for analysis

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[DOI] [PMC free article] [PubMed] [Google Scholar] 16.Leung C, Rivera L, Furness JB, Angus PW

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25 DKK-1/3/4 prevent canonical Wnt signalling by binding to LRP5/6 or further forming a tertiary complex with the single transmembrane receptors Kremen 1 and 2, which induce the endocytosis of LRP5/6

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