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Spieles-Engemann AL, Behbehani MM, Collier TJ, et al

(169) Multiscale Stem Cell Technologies for Osteonecrosis of the Femoral Head

Guinea pigs (Hartley, both sexes, 500-700 g b.w.) with whole body plethysmography, urethane anesthesia (1.5 g/kg i.p.), artificial ventilation under constant pressure (0.98 kPa), and relaxation of skeleton musculature by suxamethonium (0.2 mg/kg i.v.) were used in the present experiments

35 There is also compelling evidence that vitamin B 12 deficiency occurs frequently during pregnancy, 2 6 7 and case studies have demonstrated harmful effects of severe vitamin B 12 deficiency on the developing infant brain

Youn M, H-D C, Smith SM, Lee AG, Murphy LC, Donato M, Sweet-Cordero A, Abidi P, Bittencourt H, Lacayo NJ, Dahl G, Aftandilian C, Davis KL, Matthews JA, Kornblau SM , Huang M, Sumarsono N, Redell M, Fu CH, I-ML C, Alonzo TA, Eklund EA, Gotlib J, Khatri P, Hijiya N, Sakamoto KM

RT-PCR reactions were performed in a total volume of 25 l containing 25100 ng of total RNA, 200 m of each dNTP, 1.5 m MgCl 2 , 25 pmol of each primer, 2.5 U of AMV RT and 2.5 U of TFL DNA polymerase (Access RT-PCR System, Promega)
