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The beads were washed five times with lysis buffer, and the bound proteins were eluted with Laemmli buffer

Cellular proteomes have broad distributions of protein stability

Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: Clustal W and Clustal X version 2.0

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Mild side effects (rare) may include: Upset stomach Nausea Mild digestive discomfort Always follow label dosage and consult a healthcare professional if pregnant, nursing, or under medication