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RNA samples extracted from both colon tissue and in vitro culture experiment were reverse transcribed using the qFlex cDNA Synthesis Kit (Quanta Bioscience)

Since the M1 model is LPS induced, the presence of oxylipins and bile acids are in accordance with the immune response of pro-inflammatory AM

Consistency and proper medical guidance are far more important than timing strategies

Statements Data availability statement The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author