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Real-time qPCR Total RNA was isolated with TRIZOL reagent (Invitrogen) and reverse transcription was performed by ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO), according to the manufacturers protocol

Eng AG, Kelver DA, Hedrick TP, Swanson GT

PLoS One 9, e112394

Demyelination-induced defects in axonal transport has been also detected in MS models (Lin et al., 2014

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Signal transducers and activators of transcription as downstream targets of nongenomic estrogen receptor actions