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Figure 3 ) was also found to be much lower in the white blood cells of healthy older adults than of younger adults (17)

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Total protein (25 g/lane) was separated using 12% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) at 80110 V for 150 min and then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA, USA) at 100 V for 2 h

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