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Bone marrow cells were cultured for 6 d in a complete Roswell Park Memorial Institute (RPMI) 1640 medium containing 10 % fetal bovine serum (FBS), 10 ng/mL recombinant mouse granulocytemacrophage colony-stimulating factor (GM-CSF) (RP-8620, Invitrogen) and 10 ng/mL recombinant mouse IL 4 (404-ML/CF, RD) in 37 C, 5 % CO 2 incubator

Nevertheless, the underlying mechanisms by which NNMT regulates SIRT1 require further investigation and so does whether NNMT alters the activity of other sirtuins

After chronic CS exposure, a cascade of inflammatory processes is triggered

& Campisi, J

And it worked
