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Description
Association of bile duct and gallbladder diseases with the use of incretin-based drugs in patients with type 2 diabetes mellitus

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Samples were vortexed again, centrifuged (16,000 g , 10 min, 4 C) and then all supernatant was removed to leave a protein pellet

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REMEMBER there were only 20 patients alive at this point, probably barely alive and unable to cause problems
