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Sci Rep 16 , 13788 (2026)

(2) Because CJC-1295 appears to associate with albumin in the media via its DAC group, a small amount of free peptide may be released over time in culture

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In vitro pull-down assay showed that GSTP-S184D displayed a stronger affinity to bind HMGB1 than GSTP (WT), but GSTP-S184A did not combine with HMGB1, indicating that Ser184 phosphorylation was necessary for the association between GSTP and HMGB1 (Figures 5H,I)

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