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CircRNAs were expressed in 5,573 spermatogonial stem cells, 5,596 primitive type A spermatogonia, 6,689 preleptotene spermatocytes, 4,677 pachytene spermatocytes, and 7,220 round spermatids, according to the cell type

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The column was then washed with 10 CV of 25 mM imidazole (Sigma-Aldrich) in DPBS (pH 7.2), followed by elution with 10 CV of 250 mM imidazole in DPBS (pH 7.3)

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