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Three-dimensional image acquisition The samples were imaged with Zeiss LSM880 microscope, using a plan-Apochromat 63x/1.4 Oil DIC, and a 405-nm diode laser, 488 nm line of argon laser, 561-nm DPSS diode laser, 633-nm HeNe laser: with 70-nm xy pixel size, and 210-nm z -spacing

For transgenic constructs that did not yield stable extrachromosomal arrays after three independent trials, the plasmid was linearized by restriction enzyme digestion and amplified from promoter to 3UTR

Galvo WS, Pinheiro BB, Golalves LRB et al (2018) Novel nanohybrid biocatalyst: application in the kinetic resolution of secondary alcohols

Nature Medicine , 19(5), 576-585

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I gripped the handle tighter and tighter as I pictured pressing it against Tommys freckled, stout neck