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Therefore, we studied cell cultures under ultralow attachment conditions in which cancer cells form 3D tumor colonies or spheroids and mimic soft agar experiments, but allow easier experimental manipulation

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Commonly, transporter binding and utilization by the nanocarriers has been evaluated by the inhibitory effect of a competing substrate or inhibitor of the targeted transporter (Table 3)

The enzyme gamma-glutamylcysteine synthetase , which is the first step in creating glutathione, requires magnesium to function

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