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Description
OCR was measured following sequential injections of 2.5 M oligomycin A, 2.5 M BAM15, and 0.5 M rotenone/antimycin A (all Agilent, Seahorse XF T Cell Metabolic Profiling Kit, 103772-100) according to the manufacturers instructions

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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

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If you are taking prescribed medication, have any medical condition or are pregnant or breast-feeding please consult your healthcare practitioner before taking food supplements.
