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In consideration of the current wide use of questionable procedures, frequently including the use of commercially available, yet non-binding kits, by many researchers in the area of oxidative stress, our focus in the present article was on the emphasis of potential but avoidable pitfalls in the analysis of GSH and GSSG in biological samples, notably whole blood and RBCs

Dizhoor, A

doi: 10.1016/j.cellsig.2022.110539 208 PuriA.MohiteP.KhanS.SinghS

After that, the mixture was transferred into the column and pre-washed with wash buffer (PBS, contained 0, 10, or 30 mmol/L imidazole, pH = 7.6), followed by elution buffer (PBS, contained 300 mmol/L imidazole, pH = 7.6)

Rudman D, Kutner MH, Rogers CM, Lubin MF, Fleming GA, Bain RP

Animal species: Cattle, buffalo, horses, pigs